New method for studying the relationship between morphological parameters and cell viability

摘要

Based on the morphological differences between normal, apoptosis and necrosis cell, a new method for detection cell viability is presented in the paper. The Jurkat cells samples were used for studying the relationship between morphological parameters and cell viability in the paper. According to the scatter charts of Jurkat cell roundness, radius ratio and area, the cell areas were mainly distributed from 40 μm~2 to 80μm~2. The percentage of main areas in the two cells samples were analyzed statistically with values of 62.37% and 75.45% respectively. Due to the mostly normal cell used by the experiment in the exponent growth period, a conclusion that the areas of normal cells were mainly distributed from 40 μm~2 to 80 μm~2 is presented. And the areas of apoptosis and necrosis cells are distributed in other range, i.e. less than 40 μm~2 or more than 80μm~2 . There are not any chemical medicaments needed to add in the samples by the method based on the videopicture in order to detect the states of cell samples. What it reflected by analyzing morphological parameters of cell are the true states of cell.