The free and carrier-bound yeast populations from a two-stage immobilised yeast reactor are in different physiological conditions

摘要

To better understand the operation of a 2-stage immobilised yeast reactor making beer from wort, different yeast populations from the first stage were analysed. Yeast amounts (mg fresh yeast/g wood chip) were about: free, 10; loosely bound, 78; tightly bound, 40. Proteomics showed that from the 200 most abundant soluble proteins only 19 were present at different levels (up to 12-fold) in free and loosely bound yeast. Maltose permease activity was two-fold greater in free than loosely bound yeast. Other differences will be reported.