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Assembly, Calibration and Application of a Hyperspectral Image System for Biomedical Imaging

机译:用于生物医学成像的高光谱图像系统的组装,校准和应用

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The use of light-mater interaction for the detec-tion of chemical-physical processes is not a new concept in biology, though it is not fully developed for tissue diagnosis. It is already known that reflectance as well as fluorescence im-ages can provide important information on biochemical com-position and metabolic processes in biological samples. In spite of this potential, the experimental available image systems usually are complex making clinical implementation difficult. In this study, we describe the construction and characteriza-tion of an experimental device to produce hyperspectral im-ages between 400 and 1.000 nm. The system is composed by a spectrograph, a set of lenses for image formation and a CCD camera for acquisition. We describe in details also the calibra-tion procedure to determine system parameters as image width, field of vision, spectral and space resolution. The illu-mination system uses high power light emitting diodes, either at white light or centered at 470 and 405 nm. We demonstrate that our system is able to obtain reflectance as well as fluores-cence images of biological samples. As example of application it was used in Dentistry. Data from spectral images of violet-induced autofluorescence of bovine tooth were evaluated in order to detect demineralization process. Our results demon-strate that this system is operating as projected and it has enough sensitivity to detect changes in low-intensity optical signals as fluorescence.
机译:尽管尚未完全开发用于组织诊断的光-质相互作用用于化学-物理过程的检测,但在生物学上并不是一个新概念。众所周知,反射率以及荧光图像可以提供有关生物样品中生物化学成分和代谢过程的重要信息。尽管有这种潜力,但可利用的实验图像系统通常很复杂,使得临床实施困难。在这项研究中,我们描述了产生400至1.000 nm之间的高光谱图像的实验设备的构造和表征。该系统由一个光谱仪,一组用于成像的透镜和一个用于采集的CCD摄像机组成。我们还将详细描述用于确定系统参数(例如图像宽度,视野,光谱和空间分辨率)的校准程序。该照明系统使用高功率发光二极管,该二极管在白光下或在470和405 nm处居中。我们证明了我们的系统能够获取生物样品的反射率以及荧光图像。作为应用示例,它已在牙科中使用。为了检测脱矿物质的过程,对紫罗兰色引起的牛牙自发荧光光谱图像的数据进行了评估。我们的结果表明,该系统按预期运行,并且具有足够的灵敏度以检测低强度光信号(如荧光)的变化。

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