Robust Design And Operation Of Quasi-Continuous Adenovirus Purification By Two-column, Simulated Moving-Bed, Size-Exclusion Chromatography

摘要

Adenoviruses (Ads) are considered one of the most suitable platforms for production of viral vaccines and gene therapy vectors. Their broad tissue tropism and large transgene packing capacity make them attractive candidates for innovative virotherapies. Ads can be produced in a complementary cell line in both adherent and suspension culture systems, such as HEK-293 or PER-C6 cells, or A549 for oncolytic therapies.