The Research Progress of Bioinformatics-Led Design of Single-Chain Antibody Molecules

摘要

The development of single-chain antibody (scFv) by recombinant gene expression is an important milestone for cancer gene therapy. Single-chain antibodies are reconstructed for cancer-targeted therapy to provide good penetration into tumor tissue and to improve their pharmacokinetics in vivo, offering a clinically valuable application. However, there may be some variation between the structure and function of the fusion proteins. Analyzing the interaction region between the antibody and the antigen, and the binding sites for molecular conformation, it is clear that the existing antibodies need to be modified, enhancing the biological activity of the antibodies. Based on the view that bio-molecular computer models are closely integrated with biological experiments, a bio-molecular structure-activity relationship model can be established in terms of molecular conformation, physical and chemical properties and the biological activity of single-chain antibodies. On the one hand, the structure-activity relationship is clear for new immune molecules at the gene expression level. On the other hand, a single-chain antibody molecule can be designed and optimized for the cancer-oriented treatment. In this review, we provide the theoretical and experimental basis for the development of single-chain antibodies appropriate for cancer therapy.