Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors

摘要

We have demonstrated fluorescence resonance energy transfer (FRET) between lanthanide-ion doped oxide nanoparticles acting as donors and organic acceptor molecules (Cy5). Due to the long nanoparticle lifetime and the large Stokes shift between nanoparticle absorption and emission, unambiguous and precise FRET measurements can be performed despite the presence of large free acceptor concentrations. We determined FRET efficiencies as a function of Cy5 concentration which are in very good agreement with a multiple acceptor - multiple donor calculation.