Use of two different fluorophores for configuring fluorescence resonance energy transfer pair, where the first fluorophore acts as donor fluorophore and the second fluorophore acts as acceptor fluorophore

摘要

Use of at least two different fluorophores for configuring a fluorescence resonance energy transfer pair (FRET pair), is claimed, where: at least one first fluorophore (A) serves as the donor fluorophore and at least one second fluorophore (B) serves as the acceptor fluorophore within the FRET pair, the first fluorophore (A) and the second fluorophore (B), independently of each other, each are configured as the basis of an organo-metal complex of rare earth element, and the fluorophores (A) and (B) comprise different rare earth elements from each other. Independent claims are included for: (1) FRET complex comprising at least a first fluorophore (A) as a donor fluorophore and at least a second fluorophore (B) as an acceptor fluorophore, where: the fluorophore (A) and (B) are coupled and/or bonded over a bivalent and/or double bonded organic residue, preferably a linker and/or spacer, the first fluorophore (A) and the second fluorophore (B) forms an organometal complexes of rare earth elements and the fluorophore (A) and (B) contains different rare earth elements; (2) a FRET system comprising at least one first fluorophore (A) as a donor fluorophore and at least one second fluorophore (B) as an acceptor fluorophore; (3) a use of the FRET complexes or FRET system for the detection of an event in a sample; and (4) a FRET pair comprising at least two different fluorophores, where the FRET pair comprises the first fluorophore (A) as a donor fluorophore and the second fluorophore (B) as an acceptor fluorophore.