Nanobiomimetic Memristor/Memcapacitor Devices Used for Direct and Reagent-less Detection of Sub pM Acetyl Coenzyme A in Milks

摘要

Milk quality is key to the wellness of human babies, and detecting essential benefits and critical deficiencies in different types of milk is a crucial element to assuring good brain development. Acetyl co-enzyme A (AcCoA) is a leading substrate in a large variety of enzyme-catalyzed reactions. Unbalanced regulation of AcCoA leads to many diseases, such as diabetes, cancer, coronary disease, schizophrenia, sudden infant death syndrome and Alzheimer's. We developed a biomimetic memristor/memcapacitor sensor for direct measure sub pM concentrations of AcCoA under the conditions of free from antibody and labeling tracers, and is reagent-less. The signal changes of AcCoA over a wide concentrations range between 2 pM to 0.3μM were measured by the chronoamperometry (CA) method; it has a linear range from 2 pM to 0.4 nM. The value of Detection of Limits (DOL) is 1.2×10~(-12)M/cm~2. AcCoA spiked milk specimens were measured with a recovery value of 103%, and the method produced an error of less than 2% (n=12) by using milk samples. We studied the energy impact of human milk and USDA certified organic milk for infants upon the neuronal synapse action / resting potential pulses with and without AcCoA at slow-wave-sleeping (SWS) and 250 Hz, respectively. The results indicate that human milk has a 5-fold higher energy outcome for infants than organic milk at SWS for benefiting brain development, and human milk is orders of magnitudes sensitive for communicating with the sensor when AcCoA occurred than in organic milk when a double step chronopotentiometry (DSCPO) method was used.