Rubisco recovery from alfalfa juice by ion-exchange chromatography in expanded bed

摘要

1. Rubisco can be separated by a simple process with good performances. 2. Even the complexity and the high conductivity of the juice, Q_(10%) obtained are good. 3. Dilution of the juice before EBC treatment could be interesting in order to reduce its ionic strength and increase the dynamic capacity of the adsorbent. 4. Fixed Rubisco is easily eluted at 0.5 M NaCI. 5. Elution in fixed bed mode at low velocity allows the recovery of high concentration Rubisco fractions with high purity. 6. Experiments without centrifugation step 7. Scale up 8. Rubisco binding mechanisms.