Optical fine-needle aspiration biopsy in a rat model

摘要

Fine needle aspiration biopsy technique and following histological examination show its effectiveness and safetybut its performing takes several time. However, the problem of real-time analysis of pathological changes intissues remains relevant. We demonstrate optical fine-needle biopsy method, combining a fine needle (17.5G)and a fiber-optic probe (1 mm diameter) for minimally invasive interrogation of tissue in vivo. During rattumor experiment, we collected spectrally-resolved diffuse reectance and uorescence. Quantfued differencesbetween tumor and normal tissues were demonstrated and approved with morphological analysis. The proposedmethodology seems promising for developing new diagnostic criteria for clinical practice.