Respiration of Tribolium castaneum (Herbst) at Different Oxygen Concentrations

摘要

The main content of this research is the respiration rate of the egg, larvae of 7-10d instars（young larvae), larvae of 18-22d instars（old larvae), pupae and adults of Tribolium eastaneum (Herbst) exposed to atmospheres containing 21%, 15%, 10%,5%,3%,2% and 1% oxygen in nitrogen at 30℃. The respiration rate (RR) of them was determined with Warburg respiration instrument. The results indicated that respiration rate of the five stages was different at different oxygen atmospheres. At 21% and 15% oxygen atmospheres, RR of egg was the least, followed by young larvae, adult, pupae and old larvae. At 10% and 5% oxygen atmospheres, RR of young larvae was the least followed by egg,adults,pupae and old larvae. At 3%,2% and 1% oxygen atmospheres, RR of young larvae was similar to egg, followed by adults, pupae and old larvae. Obviously, the downtrend of RR of the five stages was different, RR of old larvae was the highest, RR of egg and young larvae was the least,and the value of them was at the same level. With the reduction of, the model of RR at the different stages there was a decrease of the logarithm,which is y(egg) =0.44ln(x)+0.12,y（young larvae) =0.52ln(x)+0.01,y(old larvae) =0.90ln(x)+1.22,y(pupae) =0.63ln(x)+0.57 and y(adult) =0.36ln(x)+1.08. The decreasing rate of the RR of the five stages was different,the reduction in the rate of old larvae was the greatest, followed by pupae, young larvae and egg and the last was the adult.At reduced oxygen levels, the decrease of respiration rates of all stages was proportional to the oxygen levels. Under 10% oxygen atmospheres,RR of two stages of larvae were significantly different (a =0.01). Under 5% oxygen atmospheres, RR of egg and adult were significantly different （a =0.01). And under 3% oxygen atmospheres, RR of pupae was markedly restrained and differed significantly (a=0.01). Hence,the reduction of RR and the reduction in degree of RR were related to the death of the insects in low oxygen atmosphere.