The Healing Effect of Recombinant Human Epidermal Growth Factor Loaded Biodegradable PLGA Microspheres on Diabetic Ulcer

摘要

Recombinant human epidermal growth factor (rhEGF) loaded microspheres were prepared by the double emulsion-solvent evaporation method using Poly (d,l-lactide-co-glycolide) (PLGA) as the matrix. The microspheres were characterized for morphology by transmission electronic microscopy (TEM), and encapsulated rhEGF was measured by enzyme-linked immunosorbant assay (ELISA). The proliferation effects of microspheres on fibroblast were evaluated by MTT assay. Finally, the rhEGF loaded microspheres were used to treat diabetic ulcer model rats. The result showed that the microspheres were smooth and spheriacal with a mean particle size 193.5nm, the polydispersity index was 0.176, and the encapsulation efficiency was 85.6%. When rhEGF loaded microspheres were applied topically once a day for 21day, there was significantly enhanced wound healing from the 14th to 21st day compared with the control (rhEGF, the blank microspheres and PBS treatment) groups. The histological and immunohistochemical examination at the postoperative 14th day revealed that the number of PCNA in the epidermis layer with the microspheres treatment were significantly higher than those of the control groups.