Preparation and the Skin Permeation Characteristics ofrhEGF (recombinant human epidermal growth factor) loaded Nano-liposome

摘要

rhEGF loaded nano-sized liposomes were preparedusing high pressure homogenization method. Particle size,zeta-potential, encapsulation efficiency and the skinpenetrated amount of rhEGF were measured. TEM(transmission electron microscopy) and CLSM (confocallaser scanning microscopy) image analysis wereperformed for the observation of morphology and skinpermeation profile of rhEGF loaded nano-liposomethrough rat skin. The mean particle size, zeta-potentialvalue and encapsulation ratio were 155.8 ± 7.76 nm, -57.92 ± 4.35 mV and about 10 %, respectively. The TEMimage analysis clearly revealed that the outer shape ofliposome was spherical and the vesicular size is rangedabout 150-200 nm. In CLSM image analysis, it seemedthat the rhEGF encapsulated into nano-liposome mainlypermeates rat skin via hair follicles and the skinpermeability of rhEGF was increased in the order of freerhEGF solution, mixture of rhEGF and liposome andrhEGF loaded nanoliposome.