Development of automated competitive ELISA paper-based analytical device using dissolvable sucrose valves for Aflatoxin B1 detection

摘要

Paper-based analytical devices are recognized as a powerful analytical tool for fluid handling and analysis. Achieving the goal of simple, inexpensive, user-friendly and on-site detection for paper-based device is especially difficult in competitive enzyme linked immunosorbent assay (ELISA) for low molecular weight compounds detection, which requires sequential delivery of reagents for sequential steps of reaction. Therefore, the device needs a special paper design to automate the fluid travel. In this study, a new paper-based device is proposed using a novel concept of dissolvable sucrose valves along with hydrophobic barriers fabricated by laser cutting machine. This method provides low cost, easy and quick device fabrication. It also prevents direct contact of reagents on the devices, resulting in automatic multistep sequencing of competitive ELISA. As a proof-of-concept demonstration for the method developed herein, the device is used to determine the levels of Aflatoxin B1 (AFB1), a carcinogenic agent.