Measurement of DNA Interstrand Crosslinks Induced by Nitrosourea

摘要

Recently,cancer has becomes a major health issues in the world because of its high mortality and incidence.Although there have proposed many pathogenic mechanism of cancer,its real mechanism has not yet been clearly.Epidemiological studies have shown that about 80％-90％of human cancers are caused by the environment,the most important cancer-causing factors is chemical factors.Nitrosourea is one kind of alkylating-agent anticancer drugs which are best represented by 1,3-Bis(2-chloroethyl)-1-nitrosourea (BCNU,carmustine),1-(2-chloroethyl)-3-cyclohexyl-nitrosourea (CCNU,lomustine),and methyl-CCNU (Me-CCNU).