6,7-Dimethoxycoumarin attenuated cisplatin-induced DNA interstrand crosslink and DNA-protein crosslink In primary cultured rabbit kidney proximal tubular cells

摘要

AIM: To study the mechanism of cisplatin interaction with DNA, and the attenuating effects of 6,7- dimethoxycoumarin (DMOC) on crosslink. METHODS: Primary cultured rabbit kidney proximal Tubular cells (PTC) were established. DNA interstrand Crosslink was assayed with ethidium bromide binding And DNA-protein crosslink with ~125I-postlabelling. CONCLUSION: The Mechanisms of cisplatin interaction with DNA in PTC Were DNA interstrand crosslink and DNA-protein Crosslink, and DMOC attenuated these effects in vitro.