Effects of CpG ODN on CD4+ and CD8+ T subpopulations in the immune response to porcine reproductive and respiratory syndrome killed virus vaccine

摘要

CpG ODN is a noval immunostimulatory reagent. In this research,the effects ofimmunostimulatory CpG oligodeoxynucleotides (CpG ODN) on CD4+ and CD8+ T lymphocytessubpopulations in the newborn piglets blood were tested at different time with porcinereproductive and respiratory syndrome killed virus vaccine (PRRS vaccine) with or without CpGODN. The results suggested that,the CD4+/CD8+ ratio decreased with age in piglets inoculatedwith vaccine alone or GpC ODN with vaccine or phosphate buffer saline (PBS),however,it wasstable in piglets co-inoculated with CpG ODN and PRRS vaccine (p>0.05),the use of CpG ODNcan prevent effectively the reduction of the proportion of CD4+ T lymphocytes. High titers ofPRRS specific antibody can also be tested in the newborn piglets serum immunized PRRS vaccineand CpG ODN (p<0.05).