A multifunctional copolymer-anticancer conjugatechitosan-graft-polyethyleneimine-candesartan (CPC) containing low molecularweight chitosan(CS)backbone and polyethyleneimine(PEI)arms with candesartan(CD)conjugated via an amide bond was fabricated as a targeted co-deliverynanovector of drug and gene for potential cancer therapy. Here, CD was utilized tospecifically bind to overexpressed angiotensinⅡtypeⅠreceptor (AT1R) of tumorcells, strengthen endosomal buffering capacity of CPC and suppress tumorangiogenesis. The self-assembled CPC/pDNA complexes exhibited desirable andhomogenous particle size, moderate positive charges, superior stability, and efficientrelease of drug and gene in vitro. Flow cytometry and confocal laser scanningmicroscopy analyses confirmed that CD-targeted function and CD-enhanced bufferingcapacity induced high transfection, specific cellular uptake and efficient intracellulardelivery of CPC/pDNA complexes in AT1R-overexpressed PANC-1 cells. In addition,CPC/wt-p53 complexes co-delivering CD andsynergistic angiogenesis suppression, aswild type p53 (wt-p53) gene achievedcompared to mono-deliverymixed-deliverytumor-targetingsystems.These findings suggested that CPC could be anandidealnanovector for simultaneous transfer of drug and gene.
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