METHOD AND DEVICE FOR DETECTING A PRESENCE OF A FLUORESCENCE PATTERN ON AN IMMUNOFLUORESCENCE IMAGE OF A BIOLOGICAL CELL SUBSTRATE

摘要

A method is proposed for detecting a presence of a fluorescence pattern on an immunofluorescence image of a biological cell substrate, comprising the following steps: incubating the cell substrate with a liquid patient sample, which potentially comprises primary antibodies, and furthermore with secondary antibodies, which are marked using a fluorescence stain, irradiating the cell substrate using excitation radiation and capturing the immunofluorescence image, determining respective items of location information, which indicate respective locations of respective relevant subsections of the cell substrate in the fluorescence image, and determining respective first partial confidence measures of respective presences of the fluorescence pattern on the respective subsections by means of a first neural network on the basis of the overall fluorescence image, extracting respective image subsections, which correspond to the respective subsections of the cell substrate, from the fluorescence image on the basis of the items of location information, determining respective second partial confidence measures of respective presences of the fluorescence pattern on the respective subsections by means of a second neural network on the basis of the respective image subsections, determining a confidence measure of the presence of the fluorescence pattern in the fluorescence image on the basis of the first partial confidence measures and the second partial confidence measures.