METHOD FOR SIMULTANEOUS DETECTION OF LYMPHOTROPIC HERPESVIRUSES HHV6A, HHV6B, EBV AND ITS MAIN GENOTYPES EBV-1 AND EBV-2

摘要

FIELD: molecular biology.;SUBSTANCE: method for the simultaneous detection of lymphotropic herpesviruses HHV6A, HHV6B, EBV and its main genotypes EBV-1 and EBV-2 in clinical samples using one-round multiplex PCR with electrophoretic detection is described. PCR is carried out in one round according to a single amplification protocol: initiation 95°C - 1 min 30 s, 45 cycles: 95°C - 30 s, 60.5°C - 40 s, 72°C - 1 min 30 s, final elongation 72°C - 8 min, in which pairs of specific primers are used: amplifying a 497 bp fragment of EBV-1, amplifying a 162 bp fragment of EBV-2, amplifying 206 bp fragment HHV6A and 431 bp fragment HHV6B.;EFFECT: simultaneous determination of HHV6A, HHV6B, as well as the main EBV genotypes (EBV-1 and EBV-2) in one sample, reducing time and simplifying the research procedure.;1 cl, 2 dwg, 2 ex