PRODUCING ISOTHIOCYANATES FROM CALLUS SUSPENSION CULTURES

摘要

A method of producing isothiocyanates includes forming, in a semi-solid or solid callus induction medium, compact callus aggregates from cells obtained from explant material of a Brassica oleracea L. plant. The method includes transferring cells from the callus aggregates, e.g. transferring the callus aggregates, to a suspension culture in a liquid medium in a shake flask, the liquid medium containing a plurality of elicitors. The method further involves transferring, after culturing in the shake flask, cells from the suspension culture to a further suspension culture in a bioreactor containing the elicitors. The method includes extracting and/or purifying of at least one isothiocyanate from cells obtained from the bioreactor. The elicitors have chitosan and salicylic acid to increase accumulation of benzyl isothiocyanate or any other isothiocyanate.