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PRODUCING ISOTHIOCYANATES FROM CALLUS SUSPENSION CULTURES
PRODUCING ISOTHIOCYANATES FROM CALLUS SUSPENSION CULTURES
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机译:从愈伤组织悬浮培养物中生产异硫氰酸盐
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摘要
The present invention provides a method of producing isothiocyanates, The method comprises forming, in a semisolid or solid callus induction medium, compact callus aggregates from cells obtained from explant material of a plant of a Brassicales plant species. The method comprises transferring cells from the callus aggregates, e.g. transferring the callus aggregates, to a suspension culture in a liquid medium in a shake flask, the liquid medium containing at least one elicitor and/or adsorbent. The method comprises transferring, after culturing in the shake flask, cells from the suspension culture to a further suspension culture in a bioreactor containing the at least one elicitor and/or adsorbent. The method comprises extracting and/or purifying of at least one isothiocyanate from cells obtained from the bioreactor.
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