ISOTHERMAL NUCLEIC ACID AMPLIFICATION METHODS FOR POINT-OF-NEED DIAGNOSIS OF EMERGING INFECTIOUS DISEASES

摘要

The present invention relates to a method for fast isothermal amplification of nucleic acids, for example by recombinase polymerase amplification (RPA) , including DNA and RNA, using thermostable enzymes. Particularly, the invention relates to diagnostic methods for rapidly diagnosing, for example, an infectious agent such as SARS-CoV-2, in a biological probe of interest. The invention further relates to a handheld and portable diagnostic system for performing the amplification method in a non-laboratory environment. Further provided are suitable enzyme sequences, kits and uses of the method and the system.