Method for detecting nucleic acid using simultaneous isothermal amplification of nucleic acids and a signal probe and isothermal nucleic acid amplification method

摘要

How to isothermal amplification of nucleic acids, and more particularly, relates to a detection method of nucleic acids comprising the step of simultaneously amplifying the amplification product detection signal probe with a nucleic acid, the present invention, RNA / DNA hybrid and an external primer set How to isothermal amplification of target nucleic acid by using the primer set, and amplified product by simultaneously amplifying signal probe and target nucleic acid using a DNA-RNA-DNA hybrid probe and primer set external primer set and RNA / DNA hybrid on how to detect. In accordance with the present invention, it is possible that it can be simple as compared with such as PCR method of the conventional method, there is no risk of contamination, and to amplify the target nucleic acid to rapidly and accurately, and simultaneously amplifying the signal probe, the pathogen detection of gene changes that result in a phenotype that confirmation and detection was defined, the diagnosis of susceptibility to disease or genetic disease, been applied to the genome project and various evaluation of gene expression, it is useful for the diagnosis of diseases and molecular biology research there. [Selection Figure Figure 2