MULTIPLEX PCR CHIP AND MULTIPLEX-PCR DETECTION METHOD USING THE SAME

摘要

The present invention relates to a multiplex PCR chip capable of simultaneously detecting a plurality of target genes and a multiplex PCR method using the same, and more particularly, to a plurality of particle formation grooves spatially separated in one or a plurality of reaction chambers. and injecting a probe in a solution state into the particle-forming grooves, and then applying a physical stimulus to form probe particles, varying the planar shape of the particle-forming grooves or forming particles on the inner surface of the particle-forming grooves The shape and pattern of the immobilizer are varied, and probes containing primers that specifically hybridize with sequences of different nucleic acid molecules are injected into the particle forming grooves, so that not only the position and shape of the probe particles but also the interior of the probe particles It is to provide a multiplex PCR chip and a multiplex PCR method using the same, enabling simultaneous multiplex detection by enabling detection of a plurality of target genes based on the shape and pattern of the particle immobilizer formed in the .