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Plasmid vector for expressing a PVT1 exon and method for constructing standard curve therefor
Plasmid vector for expressing a PVT1 exon and method for constructing standard curve therefor
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机译:表达PVT1外显子的质粒载体及其构建标准曲线的方法
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摘要
A method for cloning an exon into a plasmid vector. Exons related to prostate cancer (PVT1 exon 9, PVT1 exon 4A or PVT1 exon 4B) and miRNAs (miR-1205 or miR-1207-3p) are transformed into the plasmid vector. The cloned exons or miRNAs are linearized and their concentrations quantified. Serial dilutions in conjunction with spectroscopy permit the construction of a standard curve that permits absolute quantification of the exons or miRNAs in a biological sample from a patient.
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