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Kits and methods for the enrichment and detection of RNA viruses, in particular viruses of the Coronaviridae family
Kits and methods for the enrichment and detection of RNA viruses, in particular viruses of the Coronaviridae family
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机译:用于富集和检测RNA病毒的套件和方法,特别是Coronaviridae家族的病毒
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摘要
The present invention relates to kits and methods for the detection and enrichment of RNA viruses, in particular viruses of the Coronaviridae family. The detection method comprises coupling a binding agent that specifically recognizes a protein component of the virus and binds to it, to a carrier material, incubating the carrier material coupled to the binding agent with a virus-containing sample, staining the viruses immobilized on the carrier material with a stain for the Virus,- detection of colored virus particles via a physical, chemical or biological detection means. The method according to the invention is particularly suitable for the rapid and efficient detection of SARS-CoV-2, the causative agent of the Covid-19 pandemic. With the kits according to the invention it is possible to carry out large-scale rapid tests in a large population population. The enrichment procedure in turn makes it possible to enrich viral samples, e.g. from a throat swab of a patient, for a downstream PCR.
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