Kit Tests for Rapid Detection of Viable Bacteria and Viruses

摘要

Metabolism-linked reagents for reduction of p-iodonitrotetrazolium(INT) were optimized and reaction conditions for detecting viable bacteria on the surface of a filter were defined. The test detected 10 to the 4th power - 10 to the 5th power viable bacteria within 30 minutes. The feasibility of detecting nucleic acids and bacteriophage by binding N-Methylphenazinium methosulfate (PMS) to the nucleic acid followed by catalytic reduction of nitroblue tetrazolium(WBT) was demonstrated. The sensitivity of the test was 10 to the minus 9th power gm of RNA or DNA, corresponding to 10 to the 7th power - 10 to the 8th power virions.