Histochemical and cytochemical methods for detecting ntrk fusion proteins

摘要

A material and method for detecting ntrk rearrangements via affinity staining.Samples are screened with biomarker specific reagents (antibodies, etc.) that bind to the retention sites of TrkA, TrkB, and / or trkC.The staining pattern is evaluated and the presence of the Trk fusion is determined by detecting whether or not the sample has a threshold number of cells having at least a threshold staining intensity.In some cases, the same scoring method is applied regardless of the localized pattern of staining.In other instances, the cytoplasm and / or membrane localization is scaled by a first methodology while nuclear localization is scored by a second methodology.The methods disclosed herein may be applied throughout the non endocrine solid tumor type.No selection