DNA polymerizing enzyme with increased gene mutation specificity and PCR buffer composition for increased activity

摘要

The present invention presents a DNA mutagenesis in which mutations are induced at specific amino acid positions to increase gene mutation specificity, a nucleic acid sequence encoding the polymerizable enzyme, a vector containing the nucleic acid sequence, and a host transformed with the vector. A method for detecting one or more gene mutations or SNPs in vitro using one or more templates by providing cells and utilizing the DNA mutagenesis with increased gene mutation specificity, a gene containing the DNA mutagenesis. A composition for detecting a mutation or SNP and a PCR kit containing the composition are provided. As a result, the present invention comprises a PCR buffer composition for increasing the activity of the DNA polymerizing enzyme having increased gene mutation specificity, the PCR buffer composition and / or a gene containing the DNA polymerizing enzyme having increased gene mutation specificity. Provided is a PCR kit for detecting a mutation or SNP, and a method for detecting one or more gene mutations or SNPs in vitro using one or more templates using the kit. [Selection diagram] FIG. 6d