MULTI-LAYERED PREMIX REAGENT FOR NUCLEIC ACID AMPLIFICATION REACTIONS, AND READY-TO-USE NUCLEIC ACID AMPLIFICATION REACTION KIT

摘要

The present invention addresses the problem of providing: a premix reagent for nucleic acid amplification reactions, which makes it possible to perform a nucleic acid amplification reaction in a simple manner merely by mixing the premix reagent with a specimen; and a ready-to-use nucleic acid amplification reaction kit including the premix reagent for nucleic acid amplification reactions. A nucleic acid amplification reaction kit is provided, which comprises a reaction container and a nucleic acid amplification reaction reagent contained in the reaction container, the kit being characterized in that the reagent is a multi-layered reagent premix which is cryopreserved in a layered form comprising a first reagent layer that contains a nucleic acid polymerase, a second reagent layer that contains a primer oligonucleotide, and a film that separates the first reagent layer from the second reagent layer and is composed of a substance having a solid form at a temperature of lower than 0°C and capable of being dissolved in an aqueous solution at 0 to 35°C.