首页>
外国专利>
APPARATUS AND METHOD FOR HIGH THROUGHPUT PARALLEL NUCLEIC ACID SEQUENCING ON SURFACES OF MICROBEADS
APPARATUS AND METHOD FOR HIGH THROUGHPUT PARALLEL NUCLEIC ACID SEQUENCING ON SURFACES OF MICROBEADS
展开▼
机译:高通量平行核酸测序的装置和方法在微珠表面上测序
展开▼
页面导航
摘要
著录项
相似文献
摘要
Method and apparatus for nucleic acid sequencing are provided. The method includes providing a microbead disposed in one reaction well and immobilized with two capturing oligonucleotides with different sequences, immobilizing nucleic acid templates on the microbead via annealing between the templates and the capturing oligonucleotides, amplifying the immobilized nucleic acid templates and producing a population of template clones annealed with sequencing primers. The method further includes sequentially disposing different types of nucleotide trisphosphates, detecting, by ion-sensitive field-effect transistors, ion concentration change in the reaction wells in response to incorporation of one of the nucleotide trisphosphates at 3′ end of sequencing primers, when the nucleotide trisphosphates is complementary to a corresponding nucleotide in the template clones, and sequencing the template clones by repeating the sequentially disposing and the detecting. A method for producing single-stranded nucleic acid template clones on a reaction well array is also provided.
展开▼