Method for fabrication of peripheral nerve-mimicking microtissue and its uses

摘要

The present invention relates to a method for producing a peripheral nerve-like microtissue and a use thereof, wherein adult stem cells derived from peripheral nerves are isolated and cultured, and the separated and cultured PNSC cells are suspended in suspension. culture) to form a cell-cell and cell-extracellular matrix to form a peripheral nerve-mimicking microtissue with a diameter of 100 ± 20 μm composed of about 100 to 500 cells. will be. In microtissues produced by culturing in a suspended culture environment, about 100 to 500 cells aggregate through cell-cell bonding by β-catenin, and the extracellular matrix ( The extracellular matrix (ECM) accumulates, and it has structural properties that are coupled by β1-integrin between the accumulated ECM and cells. The cells are composed of immature peripheral nerve-derived stem cells, Schwann progenitor cells, repair Schwann cells, myelinating Schwann cells, and interstitial stromal cells. . This is similar to the peripheral nerve composition and constituent cells that are regenerated after injury. Functionally, it can induce nerve tissue regeneration by secreting neuroactive factors that act centrally on nerve regeneration in peripheral nerve-like microtissues.