SGRNA OF TARGETING TETRACYCLINE RESISTANCE GENE TETA, KNOCKOUT VECTOR THEREOF, METHOD FOR ESTABLISHING VECTOR, AND APPLICATION THEREOF

摘要

An sgRNA of a specific targeting antibiotic resistance gene tetA, a CRISPR/Cas9 gene knockout vector containing the sgRNA, a method for establishing the vector, and an application thereof, i.e., an SgRNA of a targeting tetracycline resistance gene tetA, a knockout vector thereof, a method for establishing the vector, and an application thereof. The sgRNA sequence which performs targeted cutting on the tetA gene is screened out by means of the sequence analysis on the tetracycline resistance gene tetA. Experiments verify that the provided CRISPR/Cas9 knockout vector can efficiently perform targeted cutting on the 251st to 270th sequences of the tetA gene.