LIGHT SHEET FLUORESCENCE MICROSCOPIC IMAGING DEVICE FOR IMAGING TRANSPARENT DROPLET AND TEST METHOD

摘要

A light sheet fluorescence microscopic imaging device for imaging transparentized droplets and a detection method are disclosed in the present application. The imaging device comprises a light source shaping module, a light sheet generation module, a sample control module, and an image capturing module. The light source shaping module is used to shape circular light into an elliptical light spot. The light sheet generation module is used to generate a sheet-like light beam according to the elliptical light spot. The sample control module is used to control a sample to move in a direction perpendicular to an optical axis when the sample is illuminated by the sheet-like light beam. The image capturing module is used to capture fluorescent signals excited in different positions when the sample is moving, so as to acquire a three-dimensional image sequence of the sample. The present application can be used to generate elliptical light within a short optical distance, so as to generate a high and thick light sheet, such that the shape of a light beam is more applicable to in-situ closed imaging of deep-layer droplets. At the same time, since no slit is required to block laser light, the energy utilization rate of the laser light is increased by more than four times, thereby improving the clear aperture in a large field of view, reducing the length of a capturing end, and resulting in a reduced volume and higher integration level.