LIGHT SHEET FLUORESCENCE MICROSCOPIC IMAGING DEVICE FOR IMAGING TRANSPARENT DROPLET AND TEST METHOD

摘要

The present application discloses a light sheet fluorescence microscopic imaging device for imaging a transparent droplet and a test method. The imaging device comprises a light source shaping module, a light sheet generation module, a sample control module, and an image capturing module. The light source shaping module is used to shape circular light into an elliptical spot. The light sheet generation module is used to generate a sheet-like light beam according to the elliptical spot. The sample control module is used to control the sample to move in a direction perpendicular to an optical axis when the sample is illuminated by the sheet-like light beam. The image capturing module is used to capture fluorescent signals excited in different positions when the sample is moving, so as to acquire a three-dimensional image sequence of the sample. The present application can be used to generate elliptical light within a short optical distance, so as to generate a high and thick light sheet, such that the shape of a light beam is applicable to deep-layer droplet in-situ closed imaging. In addition, since no slit is required to block laser light, the energy utilization rate of the laser light is increased by more than four times, thereby improving clear aperture in a large field of view, reducing the length of the capturing end, and resulting in reduced volume and higher integration.