KIT FOR TESTING EXPRESSION OF CA199 IN CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD OF PATIENTS WITH PANCREATIC CANCER AND TESTING METHOD

摘要

The present invention relates to the technical field of molecular biology, and relates to a kit for testing the expression of CA199 in the circulating tumor cells (CTCs) in the peripheral blood of patients with pancreatic cancer and a testing method. The kit comprises 45 mL of a diluent, 1 mL of a decolorizing solution, 0.5 mL of staining solution A, 1 mL of staining solution B, 100 μL of a goat anti-CA199 primary antibody, 100 μL of a rabbit anti-goat IgG/HRP, 100 μL of 0.1% Triton X-100, 100 μL of 0.3%H2O2, 15 ml of reagent A, 50 ml of reagent B, and 60 mL of 6×PBS buffer solution. According to the testing method of the present invention, separation is performed using a membrane filter device to obtain CTCs in the peripheral blood of patients with advanced or recurrent pancreatic cancer of which tissue specimens cannot be obtained, and further the expression of CA199 in the CTCs using immunohistochemical technology.