Method and composition for generating stem cell derived dopaminergic cell for use in the treatment of neurodegenerative disease

摘要

This disclosure relates to a method for generating dopaminergic cells and evaluating their functionality.GSK3 inhibitor and TGF - on the plate of pluripotent human embryonic stem cells coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 β When cultured in a cell culture medium containing an inhibitor and a well administered fibroblast growth factor, the desired nerve cells are produced at a much higher rate.A cell culture kit useful for producing such dopaminergic cells is also described herein as well as methods for using such cells for stem cell therapy.No selection