Transgenic chickens stabilized by multiple disulfide bridges and produced antibodies with long cdr-h3s diversified by gene transformations

摘要

This disclosure, in particular, provides a transgenic chicken.In some embodiments,Transgenic chickenEndogenous immunoglobulin heavy chain gene locus(a) CDR3 is within the range of 30 to 60 amino acidsA functional immunoglobulin heavy chain gene containing a nucleic acid encoding a heavy chain variable domain containing at least two cysteine residues and(b) it is operatively coupled to the functional immunoglobulin heavy chain geneBy gene conversionA plurality of pseudogenes that provide nucleotide sequences to nucleic acids encoding the heavy chain variable domains ofIncludingFalse geneUpstream or downstream of the functional immunoglobulin heavy chain geneContains B cells.Diagram