Efficient Transformation of indica Rice Mediated by Agrobacterium and Generation of NcGDH Transgenic Genic Male-Sterile Rice with High Nitrogen Use Efficiency

摘要

Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica varieties.Here,a fast and efficient method of Agrobacterium-mediated transformation for indica rice was developed,and an NcGDH transgenic genic male-sterile(GMS)indica rice 628S was generated using this method.The regenerated transgenic plantlets can be obtained within three months,and the transformation efficiency achieved about 17%.NcGDH transgenic 628S exhibited an enhanced capacity of ammonium assimilation and more effective panicles under low nitrogen conditions.Moreover the hybrid rice lines generated by NcGDH transgenic 628S showed 8%-27%yield advantage than those by the non-transgenic 628S.Taken together,these results provide an efficient transfbnnation method for indica rice and an opportunity to improve nitrogen use efficiency(NUE)and the heterosis of hybrid rice.