A method for detecting, on a detection support, at least one microorganism present in a sample to be analysed and revealed by means of at least one cell marker includes a first taking of an image (I0, I′0) before a step of controlled release of said markerand bringing this marker into contact with said microorganism, and at least one taking of an image (Ii) following this contact step, so as to detect said microorganism according to the change in the staining kinetics thereof, by means of a comparative analysis between the different images that have been taken. An automated detection device is capable of implementing the method and a detection support is capable of being used in the method and which is part of the device.
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