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Methods of capturing cell-free methylated DNA and uses of same
Methods of capturing cell-free methylated DNA and uses of same
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机译:捕获无细胞甲基化DNA的方法及其用途
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摘要
There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
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