Methods for capturing cell-free methylated DNA and its use

摘要

Applying a sample to library preparation to enable subsequent sequencing of the cell-free methylated DNA; Adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; Denaturing the sample; Cell methylated DNA in a sample having less than 100 ng of no-cell DNA, comprising capturing the cell-free methylated DNA using a selective binding agent to the methylated polynucleotide, .