System and method for growing biofilm biofilm on solid particles for bacterial colonization in the intestine

摘要

Problem to be solved: to provide a method for growing and encapsulating a composition comprising a biofilm and a particle for use in the colonization of the subject's gut, and at least one of the bacteria in the biofilm form.The method isForming a biofilm containing a population of at least one bacterial strain attached to the particleIn thisBio filmWhen ingested by an object that requires itConstructed to colonize at least five days in the intestine of the subjectA. obtaining a population containing at least one strain of bacteriaB. inoculating a population containing at least one strain of bacteria in a growth medium containing particlesC.Enough time for the population of at least one strain of bacteria to adhere to the particlesIncubating with groups containing at least one bacterial strainIn a growing medium containing at least one strain of bacteria attached to the particlesIncluding incubation for sufficient time to form a biofilm.No selection