Recombinant gene construction of chimeric antigen receptor for treating HIV infection and its application

摘要

TECHNICAL FIELD The present invention relates to the technical field of immunotherapy for infectious diseases, and more particularly, to the construction of recombinant gene of chimeric antigen receptor (CAR) for treating HIV infection and its application. The present invention provides a single chain antibody scFv capable of recognizing gp120 on the surface of HIV virus-infected cells and obtained by tandemly connecting the light chain and heavy chain variable regions of an antibody against gp120 on the surface of HIV virus-infected cells, CAR obtained by producing the single-chain antibody in a chimeric antigen receptor (CAR), transferring the CAR-encoding gene into a plasmid vector, and transducing a CD8+ T lymphocyte with the lentiviral vector into which the CAR-encoding gene has been transferred -T lymphocytes show significant activity in inhibiting and killing HIV-infected cells in both in vitro and in vivo experiments, can be used as an active ingredient for preparing anti-HIV infectious agents, and have good application prospects. [Selection diagram] Figure 1