Optical microscope and method for determining wavelength dependent refractive index of sample medium

摘要

Using the method of the present invention, the wavelength dependent refractive index of a sample medium to be examined with an optical microscope is determined. Using an optical microscope, sample measurement is performed on a sample medium having an unknown refractive index, illumination light is irradiated onto the sample medium, and detection light coming from the sample medium is detected. Using sample measurement, the sample measurement focal position of illumination and / or detection light is measured. The refractive index of the sample medium is defined from the sample measurement focal position using a mathematical model in which the focal position of the illumination and / or detection light is defined depending on the refractive index of the medium. In addition, an optical microscope for performing this method is described.