Reduced conversion of nicotine to nornicotine in plants

摘要

The present invention relates to a mutant, non-natural or genetically engineered tobacco plant cell, which (i) encodes a functional nicotine N-demethylase and has at least 95% sequence identity with SEQ ID NO: 2. A polynucleotide comprising, consisting of, or consisting essentially of, a sequence encoded by (ii) a polypeptide encoded by the polynucleotide described in (i), (iii) encoding a nicotine N-demethylase And a polypeptide comprising, consisting of, or consisting essentially of a sequence having at least 95% sequence identity with SEQ ID NO: 3, or (iv) an isolated poly described in (i) A nucleotide-containing structure, vector or expression vector, wherein said nicotine demethylase expression or activity , Associated with the tobacco plant cell expression or activity of the nicotine demethylase is reduced as compared to the control cigarette plants cells which have not been reduced. [Selection figure] None