SPLIT INTEIN AND PREPARATION METHOD FOR RECOMBINANT POLYPEPTIDE USING SAME

摘要

Provided is a flanking sequence pair for SspDnaE, SspDnaB, MxeGyrA, MjaTFIIB, PhoVMA, TvoVMA, Gp41-1, Gp41-8, IMPDH-1, or PhoRadA split intein. The flanking sequence pair comprises: flanking sequence a and flanking sequence b, the flanking sequence a being located at N-terminus of the split intein N-terminal protein splicing region (In) and being between N-terminus exon (En) and In, and the flanking sequence b being located at C-terminus of the split intein C-terminal protein splicing region (Ic) and being between Ic and C-terminal exon (Ec).