Preparation and purification method of recombinant human fibrost growth factor receptor by using solubility-enhancing bifunctional fusion tag combined with split intein and use thereof

摘要

The present invention relates to a method for preparing a human recombinant fibroblast growth factor receptor using a solubility-enhancing bifunctional fusion tag grafted with a split intein, a purification method, and a use thereof, and more particularly produced a dual-functional tag with improved solubility by fusing the carboxyl-terminal region of the split intein with a fusion partner for improved solubility, and fusing it to the amino-terminus of the extracellular domain of human fibroblast growth factor receptor. The present invention relates to a method for preparing a recombinant human fibroblast growth factor receptor with improved solubility, and processing the amino-terminal region of a split intein therefrom to simultaneously remove a dual functional fusion tag and purify a protein, wherein the solubility is improved through the present invention By allowing the production of the extracellular domain (Ec-FGFR) of the fibroblast growth factor receptor (Ec-FGFR) and the removal of the affinity tag to occur simultaneously, the process is simplified and economical compared to the conventional method, and it is possible to secure the structural uniformity of the domain, It can be usefully utilized in the development of pharmaceutical compositions for diagnosis and treatment of various diseases.