Method for the production of a proteu00ecna,Method for the purification of a recombinant human growth hormu00c8nio milk of a transgenic non-human mamu00ecfero which produces a hormu00c8nio recombinant human growth and method for the purification of U.M hormu00c8nio recombinant human growth of the milk of a transgenic mamu00ecfero not producing a recombinant human growth hormu00c8nio

摘要

Method for the production of a proteu00ecna,Method for the purification of a recombinant human growth hormu00c8nio milk of a transgenic non-human mamu00ecfero which produces a hormu00c8nio recombinant human growth and method for the purification of U.M hormu00c8nio recombinant human growth of the milk of a transgenic mamu00ecfero 'that produces a hormu00c8nio recombinant human growth. The invention relates to a method for the production of a protein of interest, comprising the production of a transgenic non-human mammal that produces this protein in their milk.Obtaining the said milk from transgenic non-human mammal, and the purification of the protein of interest from the milk.Transgenic bovine animals were generated, which have the capacity to produce the hormu00f3nio human growth in the mammary glands.The method involves cloning a gene construct encoding a gene of hGH and a promoter of beta casein conveniently in a vector expression.It also includes procedures for transfection in somatic cells of bovine fetuses generally fibroblasts, and the nuclear transfer into oocytes of cattle enucleados,In this way, generating a transgenic embryos.The method also includes other procedures for the generation of transgenic embryos to a greater expansion of transgenic sheep, such as subclonagem of transgenic female bovine animals,The superovulation of transgenic cows and their insemination with semen or a male bovine transgenic or non transgenic.And the superovulation of transgenic cows do not and their insemination with semen of male transgenic animals.Then, the transgenic embryos resulted in transgenic cattle that produces the hormu00f3nio human growth in large amounts in their milk,The hormu00f3nio is completely purified and analyzed to meet all the requirements for the manufacturing of a biopharmaceutical product is pure.